Gene description for HSPA8 |
Gene name |
heat shock protein family A (Hsp70) member 8 |
Gene symbol |
HSPA8 |
Other names/aliases |
HEL-33 HEL-S-72p HSC54 HSC70 HSC71 HSP71 HSP73 HSPA10 LAP-1 LAP1 NIP71 |
Species |
Homo sapiens |
Experiment description of studies that identified HSPA8 in Breast cancer cells |
1
|
Experiment ID | 65 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
19415654
|
Organism | Homo sapiens |
Experiment description | Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7. |
Authors | Staubach S, Razawi H, Hanisch FG. |
Journal name |
PROTEOMICS
|
Publication year | 2009 |
Sample | Breast cancer cells |
Sample name | MCF-7 |
Isolation/purification methods | Differential centrifugation Sucrose density gradient |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [MALDI TOF] Western blotting |
EV-TRACK |
-
|
|
|
2
|
Experiment ID | 575 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | BT549 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
3
|
Experiment ID | 577 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | MCF7 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
4
|
Experiment ID | 886 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
25102470
|
Organism | Homo sapiens |
Experiment description | Examination of the specificity of tumor cell derived exosomes with tumor cells in vitro |
Authors | Smyth TJ, Redzic JS, Graner MW, Anchordoquy TJ. |
Journal name |
Biochim Biophys Acta
|
Publication year | 2014 |
Sample | Breast cancer cells |
Sample name | MCF-7 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.10-1.20 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV140013: EV-METRIC:56%
|
|
|
5
|
Experiment ID | 1029 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
26378940
|
Organism | Homo sapiens |
Experiment description | Redefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis. |
Authors | Clark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ. |
Journal name |
Anal Chem.
|
Publication year | 2015 |
Sample | Breast cancer cells |
Sample name | SKBR3B - 100 K pellet |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV150004: EV-METRIC:67%, 38%
|
|
|
6
|
Experiment ID | 1018 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Western blotting
|
PubMed ID |
22399603
|
Organism | Homo sapiens |
Experiment description | Proteomic analysis of exosome-like vesicles derived from breast cancer cells. |
Authors | Palazzolo G, Albanese NN, DI Cara G, Gygax D, Vittorelli ML, Pucci-Minafra I. |
Journal name |
Anticancer Res
|
Publication year | 2012 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV120036: EV-METRIC:44%
|
|
|
7
|
Experiment ID | 3350 |
Identified molecule | protein
|
Extracellular vesicle type | Supermeres |
MISEV | EV-like nanoparticles |
Identification method | Mass spectrometry
|
PubMed ID |
34887515
|
Organism | Homo sapiens |
Experiment description | Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
Authors | Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectrometry ELISA |
EV-TRACK |
-
|
|
|